Abstract: . . .  sanguins et de l’étude du profil d’altération de l’ADN tumoral prélevé sur la tumeur, en utilisant les marqueurs les plus altérés [21]. CONCLUSION Nos résultats préliminaires montrent que l’ADN tumoral peut être détecté dans les urines de patients présentant un cancer du rein, quel que soit le stade ou le grade. Au regard de ces résultats, notamment pour les pT1a, dont la sensibilité est dans notre étude, comparable à l’échographie, nous pouvons envisager des applications cliniques, en termes de diagnostic et de dépistage. L’identification des micro- satellites les plus performants permettra d’améliorer encore la sen- sibilité et la spécificité du test. Les études à venir devront confirmer et comprendre pourquoi l’ADN tumoral est retrouvé dans les urines à des stades précoces. Elles devront étudier si la présence d’ADN tumoral urinaire et sérique est un facteur pronostique, mais aussi, l’évaluer pendant le suivi des cancers du rein. REFERENCES 1. COULANGE C., RAMBEAUD J.J. : “ Cancer du . . .
. . .  Cancer Res., 2002 ; 8 : 1878-1881. ____________________ SUMMARY Value of urinary microsatellite analysis in the diagnosis of renal cancer . Objective : To detect the presence of tumour DNA in urine of patients with renal cancer based on microsatellite analysis. Material and Method : Blinded, comparative, experimental study conducted between July 1996 and December 2003. Preoperative urine and blood samples were collected from 69 patients with renal cancer (pT1 to pT4). The control population comprised 35 patients with a benign urological disease. Loss of heterozygosity (LOH) and allele amplification were investigated by analysing allele imbalances between urinary DNA and blood lymphocyte DNA. Twenty-six loci were analy- sed, including 23 microsatellites. We studied the sensitivity and specifi- city of this analysis as a function of stage, grade and invasion of renal cavities by the tumour. Results :The sensitivity of the test was 61% for pT1a and the global sen- sitivity was 62.3%. A significant . . .
. . .  molecular serum analysis in renal can- cer. Clin. Cancer Res., 2002 ; 8 : 1878-1881. ____________________ SUMMARY Value of urinary microsatellite analysis in the diagnosis of renal cancer . Objective : To detect the presence of tumour DNA in urine of patients with renal cancer based on microsatellite analysis. Material and Method : Blinded, comparative, experimental study conducted between July 1996 and December 2003. Preoperative urine and blood samples were collected from 69 patients with renal cancer (pT1 to pT4). The control population comprised 35 patients with a benign urological disease. Loss of heterozygosity (LOH) and allele amplification were investigated by analysing allele imbalances between urinary DNA and blood lymphocyte DNA. Twenty-six loci were analy- sed, including 23 microsatellites. We studied the sensitivity and specifi- city of this analysis as a function of stage, grade and invasion of renal cavities by the tumour. Results :The sensitivity of the test was 61% for pT1a . . .
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